FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592244
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC576683.621806708017113No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA544253.4181318943579No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA155030.9736573037800739No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG138280.8684598591673136No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA64700.40634475620570715No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA47440.2979442849211553No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA41520.26076405375055584No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA35810.22490271591539987No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA32530.20430285810466237No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC31480.19770839142744454No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28350.17805060028488096No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAG27160.1705768713840341No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA23320.1464599646787804No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG23250.14602033356696587No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC22200.13942586688974806No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC20900.13126128909890694No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT16950.10645353350365899No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG16890.1060767068363894No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC16480.10350172461004721No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16190.1016803957182442No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT16120.10124076460642968No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)