FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091469
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC364073.335596338512592No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC273492.5057056132606608No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG94870.8691955520495773No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC66260.6070717537557182No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT48250.4420647769199125No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC36960.33862620010279726No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA33350.3055515090213281No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC30070.27550026615506257No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC23510.2153977804225315No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA20320.18617111434223052No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTC19670.18021583755470838No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA17010.1558450125473101No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC15940.1460417107586198No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTC14480.13266524289741624No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCT13620.12478595360931002No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA13610.12469433396642507No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT12280.11250892146272592No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA12040.11031005003348697No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT11590.10618716610366395RNA PCR Primer, Index 29 (96% over 25bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT11380.10426315360307988No Hit
CCTTCAGACCTGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCG11120.10188104288807102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)