FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956985
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC390234.0777023673307315No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC341813.5717383240071685No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG103241.0788047879538343No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG77690.8118204569559606No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT40550.4237265996854705No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC40250.4205917543117186No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTC27050.28265855786663324No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG26690.27889674341813087No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA23850.24922020721327923No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA22710.23730779479302183No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC22280.23281451642397738No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCC19270.20136156784066628No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTC18940.19791323792953913No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC15400.16092206251926625No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTC14950.15621979445863832No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA13130.13720173252454324No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA11420.11933311389415717No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10340.10804767054865018No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTC10330.10794317570285847No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)