FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897557
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC630213.321165055911364No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC453492.3898623335162No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATG157230.8285917102885447No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG109750.5783752477527685No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC66100.34834263213173566No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC49770.26228461121325997No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATG48420.2551702004208569No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA41190.21706857817709824No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCA31140.16410574227809757No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA27910.14708385571553317No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC27290.14381649668494806No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC26470.13949515087030323No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT25640.13512110571645544No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA21950.11567504955055367No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC21920.11551695153294472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21100.1111956057182999No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20310.10703235792126403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)