FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634969
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG739954.525773883174543No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC387822.3720327419051985No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT129750.7935930283693451No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG85210.5211719610585889No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG73510.44961097121719124No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA45550.2785985544680052No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA42810.2618398269324984No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT40240.24612087446306322No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC40120.24538691559289502No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC36270.22183906850833257No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA31420.19217489750570196No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28090.17180753885853495No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGC26950.1648349295919372No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT26580.162571889742252No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24260.14838201825233382No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC22610.13829008378752136No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC21090.12899327143205772No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG20030.12250996807890548No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA18620.11388595135442936No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA18570.11358013515852594No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA17720.108381259828168No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT17690.10819777011062595No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC16860.10312122125862937No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)