FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939901
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC232292.471430501723054No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG185541.9740376912036481No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT65260.6943284452298699No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG63420.6747519153612986No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG33090.3520583550820778No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT21490.22864110156282416No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT17800.1893816476416133No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA16690.17757189321002959No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC15730.16735805153947064No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA14050.14948382861599252No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13070.13905719857729698No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC12570.13373748937388089No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA11570.12309807096704867No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC9590.10203202252152088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)