FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004049061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566907
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC697872.718719454970515No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC419371.6337561119277013No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT95030.370212087933065No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG72320.2817398526709382No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC55220.2151227138341981No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC37520.14616813152950223No Hit
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG37280.14523315414231994No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG36030.14036348025074535No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA35420.13798707939165697No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT29830.11620989774853549No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA26800.10440580823535874No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG25670.10000362303737532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)