FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720950
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA552923.212876608849763No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC534583.106307562683402No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA159910.9291960835584997No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG139800.8123420203957116No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA90200.5241291147331416No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA43680.25381330079316655No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA40220.233708126325576No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA32070.18635056218948837No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC31930.1855370580202795No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAG30010.17438042941398646No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC26470.15381039542113367No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC25950.15078880850692933No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20890.12138644353409454No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC20580.11958511287370349No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA18840.10947441819925041No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC18540.10773119497951712No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17690.10279206252360616No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA17470.10151369882913507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)