FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416344
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG473873.3457267443502428No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC456763.2249227588777867No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG156031.1016391498110627No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG110350.779118632196698No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG65590.46309371169715835No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG42440.29964471907954565No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA34520.2437261004388764No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTC32930.23250001412086332No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGT26510.1871720429500178No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT24390.1722039278593336No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC20990.1481984602610665No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19820.13993775523460403No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTC19440.13725479120891534No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA18710.13210067610693446No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTC15570.10993092073677017No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)