FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510549
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC1221258.084808900605012No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC497343.2924453294795466No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT256491.6979919221422144No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG124290.8228134274359853No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC112950.7477413840928033No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC91420.605210423495034No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA81460.5392741314581652No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT75750.5014733054008841No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCT48820.3231937527349328No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC38690.25613204205888057No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA31070.20568680658489066No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA30430.2014499364138469No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC30150.19959630571401524No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20500.13571224766624584No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCT19410.12849632815618692No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA18880.12498767004579131No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTC17430.11538851106452025No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16070.10638516195105223No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC15280.10115527533367007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)