FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904848
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC285853.1590941240959807No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC282693.12417113150496No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATG83740.9254593036620515No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG68490.7569227096705745No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC35990.3977463618198858No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT32400.358071189857302No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA27800.3072339221615122No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC26790.2960718264283062No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATG24140.2667851396035577No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC22880.25286014888688485No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA20290.22423655685816843No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCA15230.16831556239279968No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC13560.14985942390324122No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA12800.141460223153502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC12790.14134970735416336No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA12250.135381854189875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.1344977277951656No Hit
CCTTCAGACCTGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCG11760.12996658002227998No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT11540.12753523243682918No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC10930.12079376867717008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.11659416830230049No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT9840.10874754654925467No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC9700.10720032535851325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)