FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844702
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC334111.8111868475233397No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC194191.052690353238626No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT83350.451834496845561No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG77430.4197425925705074No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA34410.1865341935987493No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC29950.1623568467969352No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25330.13731215123093052No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22750.12332615240835648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.11877257139635562No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19770.10717178167530582No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT18830.10207610768568581No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT18570.10066666594387604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)