FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223391
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC512134.186151442997374No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC506664.141439654207036No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT173841.4209684393623951No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG122701.0029499971799696No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC72260.5906533561224498No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT50100.4095174805111367No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA42790.34976552876390293No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA38060.31110250116275173No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC36300.2967162583344164No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCT27700.22641984451414143No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25890.21162490160545566No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC23250.19004553736295265No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19380.15841215114382892No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17460.1427180680583722No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC17350.14181892788160122No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC16900.1381406271584473No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG16440.1343805864192233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.1298848855353685No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT13650.11157512193566899No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG13580.11100294182317837No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT13270.10846900132500566No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA12840.10495418063399191No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA12810.10470896058578166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)