FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474833
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA287821.9515429882569755No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC251061.7022944292675848No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG67880.4602555001142502No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA59360.40248624759549045No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA39880.2704034965314717No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC29410.1994124080489113No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA23540.15961129158352166No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA20510.13906659262438528No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA19650.1332354239429142No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT19130.12970960101923404No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)