FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141291
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC188331.6501488226928978No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC184171.613698872592529No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG48470.4246944907127104No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT41830.366514762667891No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC40400.35398509232088926No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC29410.2576906328009246No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC25470.2231683242924022No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA18800.16472573603051283No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC14900.13055390781141707No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13400.1174108969579187No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC13290.1164470761619955No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA13220.1158337356554989No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA12200.10689648827512002No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTC11910.104355506176777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)