FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332947
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC281652.112987238052226No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC221981.6653325301005966No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG85820.643836551640838No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG75340.5652137706900574No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC40760.3057886022475012No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG22990.17247497462389727No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA20260.15199404027316915No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTC16620.12468612780553165No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT16290.12221041046643266No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA14210.10660588905635408No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCC13730.10300484565402826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)