FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674600
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC560393.3464110832437597No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC292891.749014690075242No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT182741.0912456706079063No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG144240.861340021497671No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC55030.32861578884509735No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT38460.2296667860981727No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG38350.2290099128150006No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA35970.21479756359727695No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA35120.2097217245909471No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG33400.1994506150722561No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC30900.18452167681834467No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC29930.17872924877582708No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT27740.1656514988654007No Hit
GTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAG27510.16427803654604084No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCT27010.16129224889525856No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC26510.1583064612444763No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG26330.1572315776901947No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG24310.14516899558103427No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT24220.14463155380389348No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC23600.14092917711692343No Hit
ACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCAC23030.13752537919503163No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC22860.13651021139376568No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC22240.13280783470679566No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG22010.1314343723874358No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG21730.12976233130299772No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA21700.1295831840439508No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC21610.12904574226680998No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG21350.1274931326884032No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC20740.12385047175444883No Hit
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG20170.12044667383255703No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG19640.11728173892272781No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC19520.11656514988654008No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA19090.11399737250686731No Hit
GACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCC19040.11369879374178908No Hit
GTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATG18940.11310163621163263No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC18940.11310163621163263No Hit
AGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTG18900.11286277319957005No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCC18890.1128030574465544No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG18700.11166845813925715No Hit
GTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGA18610.11113101636211632No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCT18600.11107130060910067No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC18580.1109518691030694No Hit
ATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGG18510.11053385883195987No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC18460.11023528006688164No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG18350.10957840678370953No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA17800.10629404036784905No Hit
CCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCT17660.10545801982563001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.10289024244595725No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC17200.1027110951869103No Hit
GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTG17120.10223336916278514No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC17060.10187507464469127No Hit
GGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACC16810.10038218081930013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)