FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067291
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC315782.958705732550916No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT258232.419490092205406No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA67220.6298188591490044No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG59920.5614213930408858No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT26830.2513841117370989No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25350.23751722819737076No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA19900.18645336651391234No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC19790.18542271976433794No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA19600.1836425117423458No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT17200.16115567356981367No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16120.1510365963921742No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA13820.12948670981016425No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13020.12199109708598686No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC12740.11936763263252477No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12520.11730633913337599No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTA12060.112996361816974No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC11900.11149723927213852No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10830.10147185725355129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)