FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157306
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG197471.7062902983307788No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC108880.9408056296260453No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG31090.2686411372618823No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCG28480.2460887613129112No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG28040.24228682820273983No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA27130.2344237392703399No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT23320.20150245483908316No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17860.15432392124468378No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16570.14317734462622678No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14600.1261550532011413No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC14500.12529097749428414No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12750.11016965262428433No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA12700.10973761477085575No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC12690.10965120720017003No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12290.10619490437274154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)