FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229496
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC404123.2868752724693695No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT302642.461496418044467No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC99190.8067533363264298No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG92400.7515274551523552No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT41060.33395797952982365No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC30030.24424642292451543No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA28480.2316396311984748No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG17440.1418467404529986No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA16520.13436399955754227No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC13700.1114277720301652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.10370102871420485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)