FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101497
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC408023.704231604806913No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC352383.199100860011421No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATG112421.020611041155809No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG88020.7990943234525377No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC42920.3896515378616555No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATG31410.2851573812729404No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA28360.25746779156003147No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC26950.2446670304140638No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT23310.21162109383865776No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCA19130.17367273810096623No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC15890.1442582231272532No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA15230.1382663774844598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.136087524523444No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC13840.12564718741857672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.11384506721307457No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC12310.11175699979210112No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)