FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073691
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC331853.090740259534633No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT193101.798469019485122No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC95500.889455159817862No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG93180.8678474533175747No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT38090.3547575606017001No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC23960.22315545161503636No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA21590.20108206178500146No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA18040.16801854537292388No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG15510.14445496888769674No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA12150.11316104912865992No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC12000.1117639991394172No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)