FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981697
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC43481721.941649000831106No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC27870414.063905834242066No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1247996.2975823246439795No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG570642.879552222161107No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA516432.605998797999896No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC449652.2690148897636724No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA315641.5927762922384199No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG230911.1652134508958736No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC179910.907858264911336No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC179190.9042250152268484No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA132330.6677610149281146No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA126570.6386950174522139No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC120940.610285023391568No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT111340.5618416942650668No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG103930.5244494995955487No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG82270.4151492382538804No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA76580.38643647338619375No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC69910.35277845200351016No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA66150.3338048147622972No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG64760.3267906243991892No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA54720.2761269760210567No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT43990.22198146336195695No Hit
TCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA43870.22137592174787568No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT41480.20931555126742382No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA40980.2067924612087519No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA40540.2045721419571206No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC39460.19912226743038922No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT36250.18292402925371537No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC35940.18135971341733878No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC35220.1777264637328512No Hit
GCGTAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG35170.177474154726984No Hit
CCTTCAGACCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA34090.17202428020025262No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC32960.16632209666765405No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC32010.16152822555617735No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCG31090.156885739848221No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC30770.15527096221067094No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC30150.15214233053791776No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT29790.15032570569567397No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC28080.14169673769501592No Hit
GTCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG27920.14088934887624094No Hit
TTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA26800.13523762714481577No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG26100.13170530106267506No Hit
CCTTCAGACCTGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCG25690.1296363672145641No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC24660.1244388016936999No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG23770.11994770138926386No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC23240.1172732259270716No Hit
TGTCCATGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA21870.11035995916631049No Hit
CCTTCAGACCTGGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAG21800.11000672655809643No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20110.10147868215978528No Hit
GCGTAACTACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG19980.10082267874453056No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC19870.10026759893162274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)