FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050768
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC222182.11445342834955No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT158811.511370730741705No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT75960.7228998218445937No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG44820.42654515554337397No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT44190.42054954090722213No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT30040.285886132809526No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA29000.27598861023556104No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT24010.2284995355777869No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA22360.21279673534024637No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT19370.18434135794009715No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC18250.1736824874758272No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT16320.1553149696222192No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA15450.14703531131515235No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC12870.12248184185281623No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATG12570.11962678726417249No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC12060.11477319446347814No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC11980.11401184657317315No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC11940.11363117262802064No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC11800.1122988138199869No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA11670.1110616234982413No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA11290.10744522101929256No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG11010.10478050340322508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)