FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977171
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT384383.9336001580071454No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC255772.6174538540337364No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT131401.3446981132268558No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT68990.706017677561041No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA55470.5676590893507891No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG43200.44209253037595264No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT41140.4210112661959882No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT33890.3468174966305795No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC32830.33596985583894734No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA29830.3052689856739506No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC29340.30025451021366784No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA28260.28920219695426896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.25225881652238963No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT24570.25144012665132304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.24253687430347398No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC21940.22452569714000928No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT21710.22217196376069287No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA18800.19239211970064604No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA15360.15718845524478317No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC14870.15217397978450037No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC13530.13846092444413516No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA12160.12444086040212No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC11780.12055208351455374No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC11760.12034741104678712No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC11650.11922171247407055No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA11120.11379789207825446No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA10730.1098067789568049No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT10480.10724837310972182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)