FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332777
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC15638211.733545821994227No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC797275.9820209982615244No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA351922.6405017493549185No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC224731.6861785580033268No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA205671.5431688872181917No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG178781.3414097031986598No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA122370.9181581014678374No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC114480.8589584003925639No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC76090.5709132135383489No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC74680.5603337992777486No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC60110.45101318525154627No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA46520.34904563929299504No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA42100.31588180168175173No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC36860.27656539691186144No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC32140.24115062009623517No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC25830.19380586549737877No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC25680.19268039589518726No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT25610.1921551767474979No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT24730.18555242174797434No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC22180.1664194385107186No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA21680.16266787317008022No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA20310.15238858413673104No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT20190.15148820845497785No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC19870.14908720663696928No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC16880.12665284589995174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.11929977783230053No Hit
GACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGG15570.11682374470747921No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG15190.11397255504859403No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC14840.11134645931014715No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC14830.11127142800333438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.10947067663982797No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA13870.10406842254930869No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC13480.10114220158361077No Hit
TTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA13350.1001667945950448No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)