FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627460
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC18874911.597765843707371No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG18581211.41730057881607No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG735344.518329175525052No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG394392.423346810367075No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG303631.8656679734064125No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA291041.788308161183685No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG210391.2927506666830522No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC129400.7951040271343074No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC126020.7743354675383727No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC116560.7162080788467919No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC97410.5985400562840254No Hit
GACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGG75280.4625612918289851No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA63830.39220626006169124No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC57020.3503619136568641No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC52160.3204994285573839No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGA43670.2683322477971809No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA42830.263170830619493No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG39330.24166492571245993No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA36890.2266722377201283No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG35770.21979034814987772No Hit
TCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG27020.1660255858822951No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC25660.15766900568984799No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC25660.15766900568984799No Hit
CCTTCAGACCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG22030.13536431002912513No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCG21410.13155469258845073No Hit
GCGTAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC19360.1189583768571885No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA18750.1152102048591056No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC18160.11158492374620575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17880.1098644513536431No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG17320.10642350656851782No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC16470.10120064394823836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)