FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667976
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC37851922.69331213398754No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC24730914.826891993649788No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA747774.483098078149805No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA595573.5706149249149868No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC502253.011134452773901No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG479122.872463392758649No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA314331.8844995371636042No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC229811.3777776179033752No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG188861.1322704883043881No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC154150.9241739689300086No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA128640.7712341184765248No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC114930.6890386912041899No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG104720.6278267792821959No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG88390.5299236919476059No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA62670.37572483057310174No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA60180.3607965582238593No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT54120.3244651002172693No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC53810.32260656028623913No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA52120.3124745200170746No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC50240.3012033746288916No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG47400.28417675074461507No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC44620.26750984426634433No Hit
GGCCTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC40300.24161019103392375No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT39270.23543504223082345No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA37560.22518309615965695No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC31280.18753267433104553No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG28430.17044609754576803No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC28180.1689472750207437No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC28070.168287793109733No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC28010.16792807570372714No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC25480.15275999175048083No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA25410.152340321443474No Hit
TGTCCATGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA23010.13795162520324034No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCG22690.13603313237120918No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC22600.13549355626220042No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT22510.13495398015319165No Hit
TCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA22500.13489402725219068No Hit
GTCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG22330.13387482793517413No Hit
GCGTAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG21630.12967812486510596No Hit
GTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA21250.12739991462706898No Hit
CCTTCAGACCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA19850.11900650848693267No Hit
GCGTAACTACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG19250.11540933442687426No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC17520.10503748255370582No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC17330.10389837743468731No Hit
TTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA17290.10365856583068342No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG17260.1034787071276805No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT17230.10329884842467758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)