FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543258
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT1016466.586455407974558No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC544293.5268892174866417No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT162171.0508288309537355No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA116950.7578123683791045No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT91500.5929015109592822No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC79380.5143663599994298No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG74390.4820321683088635No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT61300.39721161335304916No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC49170.3186116644138569No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA42150.2731234829173087No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC37160.2407892912267424No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT33740.2186283822925266No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT30400.19698585719302927No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA30080.1949123218541553No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA28090.18201752396553267No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA25730.16672520084133696No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22990.1489705545022284No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA20010.12966075665896434No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC19410.1257728778985756No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA19380.12557848396055618No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC18990.12305136276630349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.1198762617786527No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC18470.11968186784063325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.11786752441911852No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTA16170.10477833259247644No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC15890.1029639891709617No Hit
CAAAAACGGTGCTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGG15730.10192722150152471No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)