FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529199
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG16966311.094893470372398No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1234218.070957409728884No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG414022.707430491387975No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG261401.7093916488305316No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA208801.36542072025943No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG173761.1362811511124453No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG138020.9025640220795331No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC108720.7109604439971514No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA92500.6048918420689524No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC91770.6001181010450569No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC70970.46409917872036277No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA52520.3434477788698528No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA39550.25863213355488723No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA37610.24594575329960328No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC34490.22554291495089912No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC33550.21939590596122543No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG29230.19114582209378897No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC24980.16335349421494522No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA24120.15772963492652034No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG22630.147985971740761No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA21280.13915782053218712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.13856927711828218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.13150675615142304No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT19560.12791010195533742No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT18160.11875498218348297No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC16560.1082919881585065No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC16160.10567623965226239No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA15890.10391060941054761No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT15450.10103328605367908No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC15350.10037934892711804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)