FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093517
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA10960510.02316379169231No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC872907.982500500678087No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC296222.708874210460377No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA190531.7423597438357155No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA182791.6715789512188655No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG136601.2491803968296789No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC124521.138711149438006No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG63230.5782260358092284No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC53520.4894299768544979No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA46630.4264222686981547No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC42090.3849048528738008No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG40860.37365674241918506No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC31240.2856837159367436No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT30110.27535008600689337No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA26270.24023403385589798No Hit
GGCCTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA22640.2070383908069102No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA21400.1956988322998179No Hit
GCCCAGAGGTCAGCTGGCCCAGCACAGACACCCTCAGCCCTCCCTGAGGT21140.19332118293542763No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT21090.19286394267304488No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG20000.18289610495310088No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA16940.15491300089527643No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC16470.15061494242887855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16380.14979190995658961No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC15130.1383609033970208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15120.13826945534454427No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT14980.13698918260987256No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA14880.13607470208510705No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC13360.12217459810867139No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA13150.12025418900666382No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC11950.10928042270947776No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC11930.10909752660452467No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG11560.1057139486628923No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)