FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101615
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA955148.670361242357812No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC869457.892503279276335No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG313962.8499975036650733No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA157161.426632716511667No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA127241.155031476514027No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG106030.9624959718231869No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG98220.8916000599120382No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC76760.6967951598335171No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC72110.6545844056226541No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT65420.5938553850483155No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC51100.46386441724195837No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT33500.3040989819492291No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA28630.25989115979720684No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA28030.25444461086677284No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC27770.25208443966358485No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA26250.23828651570648546No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG20540.1864535250518557No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG20150.18291326824707363No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT19840.18009921796634942No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT18910.17165706712417678No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA15490.14061173822070325No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA15060.13670837815389225No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC14940.13561906836780543No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA13170.11955174902302527No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT11780.10693391066751996No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCG11310.10266744733868002No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC11250.10212279244563663No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC11210.1017596891836077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)