FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079454
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC896048.300863214180502No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT881688.167832997052212No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC309232.8646890001797205No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT142051.3159430601026074No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA136561.2650840146963187No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG106910.9904081137315717No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC94020.8709958923678083No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC75660.7009099044516951No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC67450.6248529349096859No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA60620.561580206289476No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC49440.4580093269375073No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA31170.2887570938641202No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA30190.27967843002110326No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA28920.2679132227959691No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC26270.24336377464903555No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT24140.22363157670451914No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT24040.22270518243482354No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA22300.20658592214212002No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG20600.1908372195572947No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT19640.1819438345682169No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA18360.17008598791611312No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC15110.13997817415100597No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT14140.1309921497349586No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA13940.1291393611955674No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC13620.12617489953254146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.12460002927405893No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT13240.12265460130769816No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA13070.12107973104921563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.11765207225134187No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC12640.11709623568952451No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG11380.1054236678913599No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)