FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047870
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC797873.8960969202146623No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA764443.732854136248883No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC230751.1267805085283733No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG114900.5610707710938683No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA110940.5417336061371083No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA99520.48596834760018953No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATC67860.3313686903953864No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC56700.2768730436990629No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC41200.20118464550972476No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG40980.20011035856768253No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC31690.15474615087871788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30810.150449003110549No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA30030.14664016758876294No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA26670.1302328761103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.1278401461030241No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG24270.11851338219711213No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT22370.10923544951583841No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA22310.10894246216800871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)