FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004049144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597341
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG29836211.487209419171375No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC2133128.212706764340915No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG675092.5991581390352674No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG380321.4642667250853854No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA364801.4045133080331No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG323371.2450040252704593No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG216710.8343532866881939No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC203990.7853801252896713No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC151700.5840588509556505No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA147800.5690434948664808No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC113770.4380248877602132No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA81920.31539947969866106No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA70520.27150843882262665No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA65400.25179597134146037No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC56010.21564361398830573No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC52670.20278430902988864No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA43010.16559242702440688No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC41300.15900877089300172No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG41070.15812324989287121No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA36280.13968131254232694No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG32130.12370343362692847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.12335692540948609No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA31780.1223559016702081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28230.1086880775377588No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26960.10379846158051638No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC26380.10156540862366552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)