FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667278
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT790514.741320883499933No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC486492.917869725384729No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT161160.9666054491212623No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA94530.5669720346576875No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT77410.46428969853857605No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG68970.4136682664798552No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC67500.4048515004696277No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT53940.32352133237528474No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC43780.26258368430459705No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA39920.23943217627774133No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA35360.21208220824601537No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC31130.18671151421658536No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25340.15198425217630174No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA20730.1243343941442279No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA20720.12427441614415832No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC20470.12277496614241896No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18650.1118589701297564No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA16780.10064308411674597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)