FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879836
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG1012475.385948561470256No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC719943.8298021742322206No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG253721.3496922071925423No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG142950.7604386765654025No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA117160.6232458576173666No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG107940.5741990258724697No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG78840.4193982879357561No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC76730.40817390453209745No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA59350.3157190308090706No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC57240.30449464740541193No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC42710.2272006706968055No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT32940.1752280518087748No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA30190.16059911609310598No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA28810.153258050170334No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT27760.14767245653344227No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA24300.12926659559663714No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT22420.1192657231801072No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA22270.1184677812319798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.11245661855608681No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC21000.11171187273783457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.1112863036988333No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC20590.10953083141295304No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC20190.10740298621794667No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC19240.10234935387980652No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA18810.10006192029517469No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)