FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277328
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA15851012.409498578282165No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC14385011.261790237119989No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG414063.241610612152869No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA202931.5887070509688974No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA181241.4188994526073178No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG180471.412871243721268No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG123360.9657660366014055No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC117650.9210633447321284No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC96770.7575971089649643No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT78790.616834517054351No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC70880.554908371224932No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT48490.3796205829669435No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA43890.3436079065048288No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA36360.28465672090488897No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC36320.28434356719652276No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG28080.21983390327308255No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA26000.2035499104380394No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG25020.19587764458306717No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC23620.18491726479024964No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA20900.16362281262134706No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT18940.14827828091140255No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT18890.14788683877594477No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC17540.13731790111858505No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA17380.13606528628512018No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCG14830.11610173737677401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.11555371838713314No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT14270.11171758545964702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.11148272017837235No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG14010.1096820863552666No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC13770.10780316410506934No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA13730.10749001039670311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)