FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686656
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG26420515.66442712681187No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1681809.97120930409046No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG646893.835340460651135No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA368622.185507892540032No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG344092.040072190179859No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG267831.587934943462093No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG210781.249691697655005No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC199251.1813315815435987No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC131600.7802420884875161No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC125560.7444315853380891No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC97010.5751617401532974No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA64640.3832435303938681No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC64590.3829470858313728No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA58210.345120759656978No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGA54670.32413248463231387No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC42160.2499620550960006No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG40260.23869716172118086No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC35670.21148355088411624No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA34990.20745190483418074No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG25310.15006023753509903No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT23690.14045543371025274No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC21570.12788618426045384No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA21530.12764902861045763No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC20540.12177942627305152No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG19610.11626555741063975No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT19410.11507977916065872No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCG19310.11448689003566821No Hit
TTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA18570.11009951051073842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)