FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942253
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG793404.0849467087964335No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC586943.0219544003793533No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA198891.0240169535070869No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG90240.46461506302217065No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA89910.46291600527840604No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG72000.3707035077304553No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC63920.3291023363073709No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA59980.3088166165787876No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC41710.21475060149218458No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG40060.20625531277336165No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC31910.16429373516220594No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCA24480.1260391926283548No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG21600.11121105231913658No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT20360.1048267141304454No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA19730.10158305843780394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)