FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690925
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA1114026.588228336561349No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC964225.702322693200467No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC294511.741709419400624No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA139710.8262341617753596No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG133800.7912828777148603No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA116020.686133329390718No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC88870.5255703239351243No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATC81760.48352233245117315No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC62200.3678460014489111No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG52830.31243254431745937No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC48200.2850510815086417No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG35920.2124281088753197No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA32590.19273474577524138No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA25750.1522835134615669No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC21050.12448807605304789No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT20470.12105800079837958No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA19530.11549891331667579No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19520.11543977408814703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.10290225764004908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)