FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279097
Sequences flagged as poor quality0
Sequence length76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC53552423.49720086507946No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA37655316.52202604803569No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC1348535.916948686256004No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG679412.981049073383011No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA518632.275594237542325No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA507232.225574427064754No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC346361.5197246979834556No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA243741.0694586496318497No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG242311.063184234808786No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC182890.8024669419511323No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC151650.6653951104318947No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG112750.4947134764338683No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC112410.4932216575248881No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA106840.46878215363365405No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG102190.4483793362020133No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT75530.3314031829272734No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT70460.3091575303727748No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC60950.2674304779480645No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA57740.2533459523662222No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC56480.24781744699764863No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA52780.23158294710580551No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA51660.22666872011151787No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA47100.20666079592048955No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC46860.2056077472788565No Hit
GTCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG45990.20179044595293663No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC41070.18020294879945872No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG40600.1781407285429273No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC40120.17603463125966118No Hit
TCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC37700.16541639078986106No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA36900.16190622865108417No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC36850.1616868435174106No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC34910.15317470033087666No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC33010.14483806525128154No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA32850.14413603282352616No Hit
GCGTAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG32540.14277584499475013No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC32090.1408013787916881No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG31520.13830038826780958No Hit
TGTCCATGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA30950.13579939774393104No Hit
CCTTCAGACCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC30620.13435145586168556No Hit
TCCTCAATGCTGTTGGACTGTCTCTTATACACATCTCCGAGCCCACGAGA30350.13316677613984837No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT30240.13268412884576655No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGG29260.12838418022576487No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC26880.11794144786290359No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC25590.11228131141412585No Hit
GCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA25350.11122826277249279No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC25200.11057010737147212No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT24700.10837625603473655No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC24650.10815687090106302No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT24460.1073232073931035No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGG23530.10324264390677536No Hit
TTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA23070.10122430067697864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)