FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906631
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT1455437.633516920683656No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC992055.2031567723382235No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC271351.4231909582924016No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA144210.756360302544121No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT139090.7295066533587254No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG135970.7131427108863749No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC113340.594451679428269No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC85460.44822516784841954No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC71780.3764755739311907No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA71660.375846191528408No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC54610.286421441799698No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA54290.28474308872561077No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA45970.2411059087993429No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA41570.2180285540306436No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA39300.2061227369113373No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC37590.19715403767168374No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC35160.18440904401533384No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA32190.16883182954646178No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA32030.16799265300941818No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG29490.15467072548385083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27250.14292225396524025No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG26260.1377298491422829No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT25600.134268245926978No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC25050.1313835765808906No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT22550.11827144318958414No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC22280.11685533278332305No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC21970.11522942824280105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.11155803089323525No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT20520.1076243908758433No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG20140.10563134660036473No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA19880.10426768472766887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)