FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533260
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC1275198.316854284335337No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1142687.452617299088217No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA264801.7270391192622254No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA154761.009352621212319No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG129210.8427142167668887No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC119400.7787328959211093No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC89890.5862671692994013No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA81160.5293296635926067No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC60420.39406232471987784No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT51150.3336029114435908No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC45560.2971446460482893No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT35440.23114148937557885No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA34720.22644561261625554No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA30100.19631373674393124No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA29970.1954658701068312No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA29600.1930527112166234No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC25490.16624708138215308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.16057289696463745No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT23650.15424650744166024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.15405084591002177No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23540.15352908182565253No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT23150.15098548191435243No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC21650.14120240533242895No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA20310.13246285691924398No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG18740.12222323676349739No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT18430.12020140093656653No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17940.11700559591980486No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT17860.11648383183543562No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC17050.11120097048119691No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC16880.11009222180191225No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC16700.10891825261208143No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG16500.10761384240115832No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGG16150.10533112453204284No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA15870.10350495023675045No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15550.10141789389927344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)