FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004049176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581513
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC660492.5585383455361255No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG626452.426677688626786No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA183080.7091965060799617No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA91770.3554892034245034No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG74000.2866536019768252No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG72260.27991336863304583No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA68870.266781534704648No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA57140.22134306509399723No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC52070.20170341966126068No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA50700.19639645432736538No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC47350.18341956829192801No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC42290.1638186598324316No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA36860.14278448336305105No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC36630.1418935329785285No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG35060.13581182817983098No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG34370.1331389770262633No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG33990.13166697204313904No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT32190.12469431685991897No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC27520.10660415035678689No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG26270.10176202870177296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)