FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549956
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC455782.940599604117794No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA394862.547556188691808No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC130700.8432497438636968No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG47570.3069119381453409No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA45780.2953632232140784No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA39880.25729762651326876No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC35910.2316839961908596No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATC32860.21200601823535636No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA28130.18148902291419886No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT28020.18077932534859054No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT27230.17568240646831265No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC26510.17103711331160368No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG22260.14361698009491883No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC19120.12335834049482695No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA17860.11522907747058626No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC15500.10000283879026244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)