FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578387
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA1393398.827936367950318No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1347498.53713316189249No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC323172.047469980429388No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA165801.0504394676337299No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG150070.9507807654269834No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA148610.9415308159532485No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC96170.6092929047185512No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG66400.42068263359999797No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC66350.42036585450843167No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC49410.31304109828578164No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA40940.25937872017445657No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG39550.2505722614289144No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC37180.23555693248867357No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA32690.20711017006602309No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT29830.18899040602843284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.1549683315942161No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG23830.15097691504048122No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT23630.14970979867421616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.1455916704838547No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA22340.14153689811180653No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC21030.13323728591277043No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT20080.12721848317301143No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC18490.11714490806120426No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA18070.11448396369204764No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC16940.10732475622265009No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC16920.10719804458602358No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC16760.10618435149301153No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA16230.10282649312240914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)