FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714508
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA1685229.829175483578963No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1270677.411280670606378No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG433512.5284804736985773No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA202101.1787638202913022No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA180101.0504471253560788No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG155790.908657177452657No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC141610.8259512349898629No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG126140.7357212681422309No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC101030.5892652586048008No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT88430.515774787869173No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC75110.43808486166293775No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT47440.27669745489668174No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA44370.25879144337617555No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA43200.2519673282364387No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT42930.25039253243496096No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA39210.22869534583682313No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC38280.2232710491872887No Hit
GTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTG34180.1993574833129971No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT32280.1882755869322278No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG30350.17701871324018317No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT27040.15771288322947458No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC26770.15613808742799684No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC26460.1543299885448187No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC26340.15363007929971748No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG23800.13881533361174167No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC22640.1320495442424299No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA22200.12948321034372542No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA21110.12312570136738936No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC20730.1209093220912355No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA19420.1132686461655472No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA17600.10265335594817872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)