FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538901
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1118857.270448196472677No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC1028336.682236219223979No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA284501.84872191258567No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA147070.9556820094340052No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG142270.9244909191689394No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC126650.8229899129313711No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA84490.54902817010321No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC76840.49931736999326143No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC54280.3527192457474522No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT54250.3525243014332956No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC39580.25719653181068824No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA31200.2027420867229276No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT27820.1807783606612771No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA25070.1629084651969165No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT21710.14107470201137046No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA21570.14016496187863936No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT20420.13269209650263403No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG19680.12788347008676973No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC19590.12729863714429973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19390.1259990083832553No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG18570.12067053046297324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.11378249802943788No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA16170.10507498533044037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)