FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2344648
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC47390620.212245079005463No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC42686118.205760523541272No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA1111794.7418205206069315No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG694342.961382689427155No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA659802.8140684657142567No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC535512.28396757210464No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA354041.5099921182198777No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC240941.0276169386620082No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG201640.8600011600888491No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC175890.7501765723468939No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA134440.5733909738263484No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC132390.5646476571323286No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG113030.48207662728051287No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG86300.3680723076555628No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA81890.3492635141820862No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG76570.32657354110297154No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC63650.2714693207679788No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT63310.27001920970653165No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA61680.2630672066766525No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA59360.2531723311985424No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC56600.24140084140561827No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC52310.2231038518361818No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA46930.20015797680504707No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC46360.1977269082608562No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA41840.1784489612086761No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC40810.1740559776989979No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG35930.15324261893469723No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC35560.15166455689724No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC34590.1475274753395819No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT34470.1470156714355417No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT33520.14296389052855696No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC33010.1407887239363862No Hit
GCATAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG31550.13456177643723066No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC31090.1325998614717433No Hit
TGTCCATGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA30610.1305526458555826No Hit
TCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA28870.12313148924699996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27850.11878115606265845No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA26870.11460142417966364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.1119144536834527No Hit
GTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA25660.10944073481392515No Hit
TGGTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCG24940.10636991138968407No Hit
GTCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG24730.10547425455761376No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC24720.10543160423227707No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC24330.10376824154414649No Hit
CCTTCAGACCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA24080.10270198341072946No Hit
GCGTAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG23860.10176367625332247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)